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Key Features
- Six different reference types available : 1 abs=1mg/ml, BSA, IgG, Lysozyme, user entered mass extinction coefficient, user entered molar extinction coefficient
- Measurement of wide variety of fluorescent dyes possible: Cy3, Cy5, Cy3.5, Cy5.5, Alexas 488, 546, 555, 594, 647 and 660
- Concentration of protein and up to two dyes calculated and displayed
- Reblank feature allows for the re-analysis
of previous sample with a new blank
- Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
- Sample ID field is key-focused for easy
bar code reader use
- Absorbance is represented as a 10mm path
equivalent
Measurement concentration range
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Sample Type
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Detection Limit
(pmol/ul)
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Approximate Upper Limit
(pmol/ul)
|
Typical Reproducibility
(minimum
5 replicates)
(SD= pmol/ul; CV= %)
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Cy3, Cy3.5, Alexa Fluor 555, Alexa
Fluor 660
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0.20
|
100
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sample range 0.20-4.0
pmol/ul: ± 0.20 pmol/ul sample range
>4.0 pmol/ul: ± 2%
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Cy5, Cy5.5 and Alexa Fluor 647
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0.12
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60
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sample range 0.12-2.4
pmol/ul: ± 0.12 pmol/ul sample range
>2.4 pmol/ul: ± 2%
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Alexa Fluor 488 and Alexa Fluor 594
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0.40
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215
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sample range 0.40-8.0
pmol/ul: ± 0.40 pmol/ul sample range
>8.0 pmol/ul: ± 2%
|
|
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0.30
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145
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sample range 0.30-6.0
pmol/ul: ± 0.30 pmol/ul sample range
>6.0 pmol/ul: ± 2%
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For more details view the user's manual.
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