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The capability to pre-select viable fluorescent-tagged
hybridization probes for gene expression in microarrays
can eliminate potentially flawed samples and improve
research effectiveness. The instrument software
facilitates the measurement of DNA concentration
and dye labeling effectiveness. The Thermo Scientific NanoDropTM 1000 Spectrophotometer measures the absorbance
of the fluorescent dye, allowing detection at
dye concentration as low as 0.2 pmol/ul.
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Key Features
- Includes analysis capability for dsDNA,
RNA and "other" (analysis constant
selectable between 15-55)
- Measurement of wide variety of fluorescent dyes possible: Cy3, Cy5, Cy3.5, Cy5.5, Alexas 488, 546, 555, 594, 647 and 660
- Concentration of nucleic acid and up to two dyes calculated and displayed
- Reblank feature allows for the re-analysis
of previous sample with a new blank
- Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
- 260/280 ratio calculated and displayed
- Sample ID field is key-focused for easy
bar code reader use
- Absorbance is represented as a 1mm path
equivalent
Measurement concentration range
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Sample Type
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Detection Limit
(pmol/ul)
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Approximate Upper Limit
(pmol/ul)
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Typical Reproducibility
(minimum
5 replicates)
(SD= pmol/ul; CV= %)
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Cy3, Cy3.5, Alexa Fluor 555, Alexa
Fluor 660
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0.20
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100
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sample range 0.20-4.0
pmol/ul: ± 0.20 pmol/ul sample range
>4.0 pmol/ul: ± 2%
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Cy5, Cy5.5 and Alexa Fluor 647
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0.12
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60
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sample range 0.12-2.4
pmol/ul: ± 0.12 pmol/ul sample range
>2.4 pmol/ul: ± 2%
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Alexa Fluor 488 and Alexa Fluor 594
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0.40
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215
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sample range 0.40-8.0
pmol/ul: ± 0.40 pmol/ul sample range
>8.0 pmol/ul: ± 2%
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0.30
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145
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sample range 0.30-6.0
pmol/ul: ± 0.30 pmol/ul sample range
>6.0 pmol/ul: ± 2%
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For more details view the user's manual.
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