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The Thermo Scientific NanoDropTM 1000 Spectrophotometer will
accurately and reproducibly measure purified protein
samples up to 100 mg/ml (BSA), without dilution.
To do this, the instrument detects the high concentration and automatically utilizes the 0.2mm pathlength to calculate the absorbance. A sample
measurement output is shown below.
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Key Features
- Six different reference types available : 1 abs=1mg/ml, BSA, IgG, Lysozyme, user entered mass extinction coefficient, user entered molar extinction coefficient
- Concentration of protein is calculated and displayed as mg/ml
- Reblank feature allows for the re-analysis
of previous sample with a new blank
- Full spectrum data is automatically archived in a text delimited file on the controller PC and easily reviewed using the Data Viewer feature
- 260/280 ratio calculated and displayed
- Sample ID field is key-focused for easy
bar code reader use
- Absorbance is represented as a 10mm path
equivalent
Measurement concentration range
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Sample Type
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Detection Limit
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Approximate Upper Limit
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Typical Reproducibility
(minimum
5 replicates)
(SD= mg/ml; CV= %)
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0.10 mg/ml
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100 mg/ml
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sample range 0.05-10
mg/ml: ± 0.10 mg/ml
sample range
>10mg/ml: ± 2%
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For more details view the user's manual.
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